Getting a high-quality image using confocal technology lies in the details.
Just as the telescope allows one to catch a broad glimpse of the universe, the microscope enables one to narrow the focus down to the smallest of worlds: molecules and atoms. Although conventional light and fluorescence microscopy allow for the examination of both fixed and small living samples, one challenge is that it tends to blur the details of key structures of interest because of the excitation of light-or out-of-focus