The Spot RT3 Slider digital camera system combines performance and FireWire connectivity. The system has optical color rendering, particularly when used with Spot Imaging software featuring Correct Color Technology/ICC color profiling. The performance of the RT3 Slider, along with the built-in flexibility of both color and monochrome imaging in one camera, makes the camera suited for core laboratory imaging needs. Low read noise and deep pixel wells of its Kodak KAI 2020 CCD make it a sensitive imaging system. With effective differential cooling of -43 C, dark current has been reduced to under 0.003 e/p/s, making the camera suited for fluorescence imaging. Spot Basic Image Capture software is included with each RT3 Slider camera and can be enhanced to include macro scripting and peripheral device control.